A Facile Surface-Imprinting Strategy for Trypsin-Imprinted Polymeric Chemosensors Using Two-Step Spin-Coating

Surface imprinting used for protein recognition in functional cavities is highly effective biomacromolecules to avoid template encapsulation during the formation of a molecularly imprinted polymer (MIP) matrix. Herein, we introduce facile surface-imprinting method based on two-step spin-coating and photopolymerization design efficient sites polymeric films detect trypsin (TRY). Well-distributed successfully achieved maximized sensing responses by controlling composition monomers crosslinkers precursor solution concentration TRY solution. The MIP film exhibits higher sensitivity (?841 ± 65 Hz/(?g/mL)) with coefficient determination 0.970 factor 4.5 0.24 µg/mL compared nonimprinted (NIP) film. Moreover, limit detection quantification are calculated be 25.33 84.42 ng/mL, respectively. Finally, selectivity within range 3.90–6.78 against other proteins. These properties superior those corresponding Thus, proposed high selectivity.